ABO 抗体
Quick Overview for ABO 抗体 (ABIN6252832)
抗原
See all ABO 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- PEG precipitation
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免疫原
- A mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A was used as the immunogen for the ABO antibody.
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亚型
- IgM kappa
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anti-Histo-blood group A transferase (ABO) (AA 54-354) antibody
ABO 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) (AA 273-354) antibodyABO 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 1B7 unconjugated
anti-Histo-blood group A transferase (ABO) (N-Term) antibodyABO 适用: 人, 小鼠, 大鼠 ELISA, IHC, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) (AA 41-68), (N-Term) antibodyABO 适用: 人 WB 宿主: 小鼠 Monoclonal 593CT3-2-2 unconjugated
anti-Histo-blood group A transferase (ABO) antibodyABO 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) antibodyABO 适用: 人 IHC, IF, AGG, H, StM 宿主: 小鼠 Monoclonal HEB-20 unconjugated
anti-Histo-blood group A transferase (ABO) antibodyABO 适用: 人, 小鼠, 大鼠 IHC, WB, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) (AA 235-354) antibodyABO 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) antibodyABO 适用: 人 IHC, IF, AGG, H, StM 宿主: 小鼠 Monoclonal HE-193 unconjugated
anti-Histo-blood group A transferase (ABO) antibodyABO 适用: 人 IHC, IF, StM 宿主: 小鼠 Monoclonal 19-OLE unconjugated
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应用备注
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Functional Activity-Erythrocyte Agglutination
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Optimal dilution of the ABO antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- ABO (Histo-blood group A transferase (ABO))
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别名
- ABO
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背景
- This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssmann antigen. It is also reactive with the immuno-dominant A trisaccharide. Blood group antigen expression in human colon cancer was studied by means of two monoclonal antibodies of broad anti-A (HE-14) and anti-type 3 and type 4 chain-based A and H (HE-10) specificity. These antigens were proved to re-appear in tumors of the distal colon, the HE-10 antibody reacting more frequently (9 out of 12 samples) than HE-14 (5 out of 12 samples) and frequently with supra-nuclear staining of the cytoplasm probably in those places of the Golgi apparatus where carbohydrate antigens are synthesized. This staining pattern is characteristic of HE-10 in normal colonic mucosa as well. With HE-14, staining was often absent in less differentiated tumors, while HE-10 did react in such tumors. In some cases, these two antibodies gave different staining patterns in parallel sections from the same tissue sample, primarily at the cellular level. Three out of 12 cases showed blood group antigen expression in the mucosa of the distal colon adjacent to the tumor only when HE-10 mAb was used.
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基因ID
- 28
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UniProt
- P16442
抗原
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