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PAX7 抗体 (AA 300-600)

This anti-PAX7 antibody is a 小鼠 单克隆 antibody detecting PAX7 in IHC (p). Suitable for 人.
产品编号 ABIN6252801
发货至: 中国

Quick Overview for PAX7 抗体 (AA 300-600) (ABIN6252801)

抗原

See all PAX7 抗体
PAX7 (Paired Box 7 (PAX7))

适用

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宿主

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小鼠

克隆类型

  • 63
  • 47
单克隆

标记

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This PAX7 antibody is un-conjugated

应用范围

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

PBPX7
  • 抗原表位

    • 22
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    AA 300-600

    纯化方法

    Protein G affinity chromatography

    免疫原

    A recombinant fragment (aa300-600) was used as the immunogen for the PAX7 antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

    Prediluted format: incubate for 30 min at RT (2)

    Optimal dilution of the PAX7 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris buffer with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    PAX7 (Paired Box 7 (PAX7))

    别名

    PAX7

    背景

    The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).

    基因ID

    5081

    UniProt

    P23759
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