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lambda 抗体

The 小鼠 单克隆 anti-lambda antibody (Clone LAM03) (ABIN6252722) specifically detects lambda in FACS, IF, WB 和 IHC. The antibody is reactive with 人 samples.
产品编号 ABIN6252722
发货至: 中国
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北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for lambda 抗体 (ABIN6252722)

抗原

lambda

适用

宿主

  • 5
  • 3
小鼠

克隆类型

  • 5
  • 3
单克隆

标记

  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
非结合性

应用范围

  • 8
  • 5
  • 5
  • 3
  • 2
  • 2
  • 1
Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)

克隆位点

LAM03
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    Purified human lambda light chain was used as the immunogen for this Lambda antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 0.5-1 μg/mL

    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

    The optimal dilution of the Lambda antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    lambda

    背景

    This mAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

    基因ID

    28820

    UniProt

    P01701, P01842
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