电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

CD235a/GYPA 抗体

This anti-CD235a/GYPA antibody is a 小鼠 单克隆 antibody detecting CD235a/GYPA in FACS, WB, IF 和 IHC (p). Suitable for 人.
产品编号 ABIN6252670
发货至: 中国

Quick Overview for CD235a/GYPA 抗体 (ABIN6252670)

抗原

See all CD235a/GYPA (GYPA) 抗体
CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

适用

  • 157
  • 17
  • 9
  • 2

宿主

  • 90
  • 71
  • 10
  • 1
  • 1
  • 1
小鼠

克隆类型

  • 103
  • 70
单克隆

标记

  • 61
  • 20
  • 13
  • 9
  • 7
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This CD235a/GYPA antibody is un-conjugated

应用范围

  • 98
  • 73
  • 58
  • 32
  • 26
  • 24
  • 20
  • 16
  • 13
  • 13
  • 13
  • 5
  • 5
  • 5
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

GYPA-280
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    Recombinant human GPA protein was used as the immunogen for the Glycophorin A antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 0.5-1 μg/mL

    Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)

    Prediluted format : incubate for 30 min at RT (2)

    Optimal dilution of the Glycophorin A antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

    别名

    Glycophorin A

    背景

    Recognizes a sialoglycoprotein of 39  kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

    基因ID

    2993

    UniProt

    P02724

    途径

    Maintenance of Protein Location
You are here:
Chat with us!