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Cadherin-16 抗体

This anti-Cadherin-16 antibody is a 小鼠 单克隆 antibody detecting Cadherin-16 in IF, FACS 和 IHC (p). Suitable for 人, 小鼠, 大鼠, 犬 和 兔.
产品编号 ABIN6252616
发货至: 中国

Quick Overview for Cadherin-16 抗体 (ABIN6252616)

抗原

See all Cadherin-16 (CDH16) 抗体
Cadherin-16 (CDH16)

适用

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人, 小鼠, 大鼠, 犬, 兔

宿主

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  • 1
小鼠

克隆类型

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单克隆

标记

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This Cadherin-16 antibody is un-conjugated

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

CDH16-1071
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    Recombinant human protein was used as the immunogen for the Cadherin 16 antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 1-2 μg/mL

    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

    Prediluted format: incubate for 30 min at RT (2)

    Optimal dilution of the Cadherin 16 antibody to be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    Cadherin-16 (CDH16)

    别名

    Cadherin 16

    背景

    This mAb recognizes a protein of 130  kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels. Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.

    基因ID

    1014

    UniProt

    O75309
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