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HCG beta 抗体

适用: 人 ELISA, WB, IHC (fro) 宿主: 小鼠 Monoclonal HCGb-211 unconjugated
产品编号 ABIN6252542
发货至: 中国
  • 抗原 See all HCG beta 抗体
    HCG beta
    适用
    • 72
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    宿主
    • 39
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    小鼠
    克隆类型
    • 41
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    单克隆
    标记
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    This HCG beta antibody is un-conjugated
    应用范围
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    ELISA, Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
    纯化方法
    Protein G affinity chromatography
    免疫原
    Purified HCG-beta protein was used as the immunogen for this antibody.
    克隆位点
    HCGb-211
    亚型
    IgG1 kappa
    Top Product
    Discover our top product HCG beta Primary Antibody
  • 应用备注
    ELISA: use BSA-free format (1)

    IHC (Frozen): 0.5-1 μg/mL for 30 min at RT

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCG-beta antibody to be titered up or down for optimal performance.

    1. Clone HCGb/211 (recommended capture) will pair with clones < a href=../tds/hcg-beta-antibody-hcgb459-v2373>HCGb/459 and < a href=../tds/hcg-beta-antibody-hcgb54-v2092>HCGb/54 (recommended detect) by ELISA.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 mg/mL
    缓冲液
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原
    HCG beta
    别名
    HCG-beta (HCG beta 产品)
    背景
    This antibody reacts with a protein of 22  kDa, identified as beta subunit of HCG. It does not cross react with the alpha subunit. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG-beta antibody detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate antibody positivity in 90 % and 60 % of cases respectively, 20 % of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.
    基因ID
    1082
    UniProt
    Q6NT52
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