HCG beta 抗体
Quick Overview for HCG beta 抗体 (ABIN6252542)
抗原
See all HCG beta 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Purified HCG-beta protein was used as the immunogen for this antibody.
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亚型
- IgG1 kappa
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anti-HCG beta (AA 81-165) antibody
适用: 人 ELISA, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-HCG beta antibody适用: 人, 小鼠, 大鼠 ELISA, IHC, WB, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-HCG beta (C-Term) antibody适用: 人 ELISA, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-HCG beta (AA 101-150) antibody适用: 人 ELISA, IHC, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: use BSA-free format (1)
IHC (Frozen): 0.5-1 μg/mL for 30 min at RT
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCG-beta antibody to be titered up or down for optimal performance.1. Clone HCGb/211 (recommended capture) will pair with clones < a href=../tds/hcg-beta-antibody-hcgb459-v2373>HCGb/459 and < a href=../tds/hcg-beta-antibody-hcgb54-v2092>HCGb/54 (recommended detect) by ELISA.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- HCG beta
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别名
- HCG-beta
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背景
- This antibody reacts with a protein of 22 kDa, identified as beta subunit of HCG. It does not cross react with the alpha subunit. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG-beta antibody detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate antibody positivity in 90 % and 60 % of cases respectively, 20 % of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.
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基因ID
- 1082
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UniProt
- Q6NT52
抗原
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