GNRHR 抗体
Quick Overview for GNRHR 抗体 (ABIN6252498)
抗原
See all GNRHR 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant full-length human GnRHR protein was used as the immunogen for the GnRH Receptor antibody.
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亚型
- IgG1 kappa
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anti-Gonadotropin-Releasing Hormone Receptor (GNRHR) (AA 201-328) antibody
GNRHR 适用: 人, 大鼠 WB, ELISA, IHC (p), IHC (fro), ICC, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Gonadotropin-Releasing Hormone Receptor (GNRHR) antibodyGNRHR 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Gonadotropin-Releasing Hormone Receptor (GNRHR) antibodyGNRHR 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Gonadotropin-Releasing Hormone Receptor (GNRHR) (Internal Region) antibodyVerified GNRHR 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Gonadotropin-Releasing Hormone Receptor (GNRHR) (N-Term) antibodyGNRHR 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Gonadotropin-Releasing Hormone Receptor (GNRHR) (AA 3-13), (Extracellular) antibodyGNRHR 适用: 大鼠 WB, IF, FACS, IHC, LCI 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Optimal dilution of the GnRH Receptor antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))
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别名
- GnRH Receptor
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背景
- Recognizes an epitope on the extracellular domain of gonadotropin releasing hormone (GnRH) receptor or luteinizing hormone receptor (LHCGR). Lutropin (also designated luteinizing hormone) plays a role in spermatogenesis and ovulation by stimulating the testes and ovaries to produce steroids. Gonadotropin (also designated choriogonadotropin) production in the placenta maintains estrogen and progesterone levels during the first trimester of pregnancy. Ovaries and testes abundantly express luteinizing hormone/choriogonadotropin receptor. GnRH receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular and intracellular loops characteristic of G-protein coupled receptors. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinizing hormone (LH) as well as follicle-stimulating hormone (FSH). GnRH influences the protective effect of pregnancy and Gonadotropin against breast cancer. The expression of GnRH on breast carcinoma correlates in part to the degree of tumor differentiation. GnRH-positive breast tumors occur more frequently in tumors with greater cell differentiation in premenopausal women. GnRH is present in luteal and granulosa cells as well as in ovarian cell membrane preparations.
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基因ID
- 2798
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UniProt
- P30968
抗原
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