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Fibronectin 抗体

This anti-Fibronectin antibody is a 小鼠 单克隆 antibody detecting Fibronectin in IHC, IF 和 FACS. Suitable for 人.
产品编号 ABIN6252186
发货至: 中国

Quick Overview for Fibronectin 抗体 (ABIN6252186)

抗原

See all Fibronectin 抗体
Fibronectin

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Fibronectin antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

克隆位点

HFN7-1
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    Purified human Fibronectin was used as the immunogen for this antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Flow Cytometry: 0.5-1 μg/million cells

    IF: 0.5-1 μg/mL

    IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

    Titration of the antibody may be required for optimal performance.

    1. FFPE staining requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    Fibronectin

    背景

    The Fibronectin dimer consists of two disulfide linked monomers of approximately 220 kD, and is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. It accumulates at the site of injury and, along with fibrin, forms a blood clot to stop bleeding and protect damaged tissue.

    The HFN7.1 Fibronectin antibody is directed against the peptide core and reacts with both the plasma and cellular forms. The antibody blocks the fibronectin-mediated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface.

    基因ID

    2335

    UniProt

    P02751
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