TOX3 抗体 (AA 200-400)
Quick Overview for TOX3 抗体 (AA 200-400) (ABIN6252144)
抗原
See all TOX3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 200-400
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纯化方法
- Protein G affinity chromatography
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免疫原
- A recombinant fragment (139 amino acid residues around aa 200-400) from the human protein was used as the immunogen for the TOX3 antibody.
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亚型
- IgG2b kappa
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anti-TOX High Mobility Group Box Family Member 3 (TOX3) (AA 214-242) antibody
TOX3 适用: 人, 小鼠 FACS, WB, IHC (p) 宿主: 兔 Polyclonal RB24743 unconjugated
anti-TOX High Mobility Group Box Family Member 3 (TOX3) (AA 8-305) antibodyTOX3 适用: 人 WB, IF, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-TOX High Mobility Group Box Family Member 3 (TOX3) (AA 251-389) antibodyTOX3 适用: 人 WB, ELISA, Coat 宿主: 小鼠 Monoclonal TOX3-1124 unconjugated
anti-TOX High Mobility Group Box Family Member 3 (TOX3) (AA 200-400) antibodyTOX3 适用: 人 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal TOX3-1123 unconjugated
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Optimal dilution of the TOX3 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 or 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- TOX3 (TOX High Mobility Group Box Family Member 3 (TOX3))
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别名
- TOX3
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背景
- It recognizes a 63 kDa protein, which is identified as TOX3. It contains a high mobility group (HMG)-box, which regulates Ca2+-dependent transcription in neurons through interaction with the cAMP-response-element-binding protein (CREB). TOX3 appears to be associated with breast cancer susceptibility and is expressed downstream of a cytoprotective cascade together with CITED1, a transcriptional regulator that does not bind directly to DNA. TOX3 is predominantly expressed in the brain and forms homodimers. TOX3 overexpression protects neuronal cells from cell death caused by endoplasmic reticulum stress or BAX overexpression through the induction of anti-apoptotic transcripts and repression of pro-apoptotic transcripts.
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基因ID
- 27324
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UniProt
- O15405
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途径
- Chromatin Binding
抗原
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