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RBP4 抗体 (AA 74-182)

This anti-RBP4 antibody is a 小鼠 单克隆 antibody detecting RBP4 in WB, IHC 和 IF. Suitable for 人, 小鼠, 大鼠, 兔, 猴 和 山羊.
产品编号 ABIN6252115
发货至: 中国

Quick Overview for RBP4 抗体 (AA 74-182) (ABIN6252115)

抗原

See all RBP4 抗体
RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

适用

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人, 小鼠, 大鼠, 兔, 猴, 山羊

宿主

  • 148
  • 53
  • 8
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  • 1
  • 1
小鼠

克隆类型

  • 157
  • 59
单克隆

标记

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This RBP4 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

克隆位点

G4E4
  • 抗原表位

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    AA 74-182

    纯化方法

    Protein G affinity chromatography

    免疫原

    Retinol binding protein purified from human plasma was used as the immunogen for this RBP antibody. Its epitope localizes between amino acids 74-182.

    亚型

    IgG1 kappa
  • 应用备注

    IHC (FFPE): 1-2 μg/mL for 30 min at RT (1)

    Prediluted format : incubate for 30 min at RT (2)

    IF: 1-2 μg/mL

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

    别名

    RBP

    背景

    Retinol binding protein a single-chain glycoprotein belonging to the superfamily of hydrophobic molecule transporter proteins, which is responsible for transport of retinol (vitamin A1) from the liver to peripheral target tissues, like the eye, where it mediates the cellular uptake. RBP1 is synthesized by hepatic parenchymal cells where it becomes bound to its ligand retinol and is then released into the circulation, where it binds further to the protein transthyretin, to form a transporting complex, which is big enough not to be lost by filtration through the kidney glomeruli. It is detected in nearly all tissues with higher expression in adult ovary, pancreas, pituitary gland, adrenal gland, and fetal liver.

    基因ID

    5947

    UniProt

    P09455

    途径

    Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response
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