MFGE8 抗体
Quick Overview for MFGE8 抗体 (ABIN6252079)
抗原
See all MFGE8 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Does not react with mouse and rat
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纯化方法
- Protein G affinity chromatography
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免疫原
- A human milk fat globule membrane preparation was used as the immunogen for the MFGE8 antibody.
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亚型
- IgG1 kappa
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应用备注
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Western blot: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Optimal dilution of the MFGE8 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))
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别名
- MFGE8
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背景
- Recognizes a protein of 40-45 kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin, or MFG-E8. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.
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基因ID
- 4240
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UniProt
- Q08431
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途径
- SARS-CoV-2 Protein Interactome
抗原
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