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MFGE8 抗体

This anti-MFGE8 antibody is a 小鼠 单克隆 antibody detecting MFGE8 in WB, FACS, IF 和 IHC (p). Suitable for 人.
产品编号 ABIN6252078
发货至: 中国

Quick Overview for MFGE8 抗体 (ABIN6252078)

抗原

See all MFGE8 抗体
MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This MFGE8 antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

SPM291
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    A human milk fat globule membrane preparation was used as the immunogen for the MFG-E8 antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Western blot: 1-2 μg/mL

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 1-2 μg/mL

    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

    Prediluted format : incubate for 30 min at RT (2)

    Optimal dilution of the MFG-E8 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

    别名

    MFG-E8

    背景

    Recognizes a protein of 40-45  kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin or MFG-E8. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.

    基因ID

    4240

    UniProt

    Q08431

    途径

    SARS-CoV-2 Protein Interactome
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