Granulocyte Marker 抗体
Quick Overview for Granulocyte Marker 抗体 (ABIN6252005)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes were used as the immunogen for this Granulocyte Marker antibody.
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亚型
- IgG1
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anti-Granulocyte Marker antibody
适用: 人 IF, FACS, IHC (f), IHC (p), ISt 宿主: 小鼠 Monoclonal BM-1 unconjugated
anti-Granulocyte Marker antibody适用: 人, Macaque IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal BM-2 unconjugated
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Granulocyte Marker antibody to be titered up or down for optimal performance.1. No special pretreatment is required for staining of formalin/paraffin tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Granulocyte Marker
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背景
- This antibody recognizes an unidentified antigen in the cytoplasm of mature granulocytes and can be used as a granulocyte marker in normal tissues or inflammatory processes. The granulocyte marker antibody shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. Clone BM-2 mAb reacts with early precursor and mature forms of human myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas.
抗原
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