Ferritin 抗体 (AA 38-165)
Quick Overview for Ferritin 抗体 (AA 38-165) (ABIN6252003)
抗原
See all Ferritin (FE) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 38-165
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纯化方法
- Protein G affinity chromatography
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免疫原
- Amino acids 38-165 of human FTL were used as the immunogen for this Ferritin antibody (light chain specific).
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亚型
- IgG2b
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anti-Ferritin (FE) antibody
FE 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ferritin (FE) antibodyKD Validated FE 适用: 人, 小鼠, 大鼠 WB, ICC, IF 宿主: 兔 Monoclonal unconjugated Recombinant Antibody
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应用备注
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Western blot: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/10^6 cells
IF: 1-2 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ferritin antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Ferritin (FE)
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别名
- Ferritin
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背景
- Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.
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基因ID
- 2512
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UniProt
- P02792
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途径
- Transition Metal Ion Homeostasis
抗原
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