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FOXP3 抗体 (N-Term)

This anti-FOXP3 antibody is a 小鼠 单克隆 antibody detecting FOXP3 in FACS, IHC 和 IF. Suitable for 人 和 小鼠.
产品编号 ABIN6251992
发货至: 中国

Quick Overview for FOXP3 抗体 (N-Term) (ABIN6251992)

抗原

See all FOXP3 抗体
FOXP3 (Forkhead Box P3 (FOXP3))

适用

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人, 小鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This FOXP3 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

克隆位点

FHBP3-1
  • 抗原表位

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    N-Term

    纯化方法

    Protein G affinity chromatography

    免疫原

    An N-terminal recombinant protein fragment was used as the immunogen for this FOXP3 antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Flow Cytometry: 0.5-1 μg/million cells

    IF: 0.5-1 μg/mL

    IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

    Prediluted format : incubate for 30 min at RT (2)

    Titering of the FOXP3 antibody may be required for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris buffer with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    FOXP3 (Forkhead Box P3 (FOXP3))

    别名

    FOXP3

    背景

    The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXP3 is essential for normal immune homeostasis. It represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.

    基因ID

    50943

    UniProt

    Q9BZS1

    途径

    Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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