S100A9 抗体
Quick Overview for S100A9 抗体 (ABIN6251943)
抗原
See all S100A9 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
纯化方法
- PEG precipitation
-
免疫原
- Recombinant human protein was used as the immunogen for the S100A9 antibody.
-
亚型
- IgM kappa
-
-
-
-
应用备注
-
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)
Titering of the S100A9 antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Not suitable for frozen tissue testing.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.2 mg/mL
-
缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
-
-
- S100A9 (S100 Calcium Binding Protein A9 (S100A9))
-
别名
- S100A9
-
背景
- S100A9, also known as Calgranulin B or MRP14, is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. [UniProt]
-
基因ID
- 6280
-
UniProt
- P06702
-
途径
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
抗原
-