Keratin 10/13 抗体
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北京 101111
Quick Overview for Keratin 10/13 抗体 (ABIN6251937)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the Cytokeratin 10 + 13 antibody.
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亚型
- IgG2a kappa
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anti-Keratin 10/13 antibody
适用: 人, Cat, 斑马鱼 WB, FACS, IHC (p), IHC, IHC (fro), ICC 宿主: 小鼠 Monoclonal DE-K13 unconjugated
anti-Keratin 10/13 antibody适用: 人, Cat WB, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal DE-K13 unconjugated
anti-Keratin 10/13 antibody适用: 人, Cat WB, FACS, IHC (p), IHC, IHC (fro), ICC 宿主: 小鼠 Monoclonal DE-K13 unconjugated
anti-Keratin 10/13 antibodyCarrier-free 适用: 人 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal DE-K13 unconjugated
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.25-0.5 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Optimal dilution of the Cytokeratin 10 + 13 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Keratin 10/13
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别名
- Cytokeratin 10 + 13
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背景
- This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this mAb serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
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基因ID
- 3858, 3860
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UniProt
- P13645, P13646
抗原
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