Nuclear Marker 抗体
Quick Overview for Nuclear Marker 抗体 (ABIN6251935)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- Does not react with Pig
-
纯化方法
- Protein G affinity chromatography
-
免疫原
- Nuclei of HL60 cells were used as the immunogen for the Nuclear marker antibody.
-
亚型
- IgG1 kappa
-
-
anti-Nuclear Marker antibody
适用: 人, 小鼠, 大鼠 FACS, IF, ICC, IHC (p) 宿主: 小鼠 Monoclonal NM106 unconjugated
anti-Nuclear Marker antibody (PE)适用: 人, 灵长类 FACS, IF 宿主: 小鼠 Monoclonal 235-1 PE
-
-
应用备注
-
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunocytochemistry (Acetone-fixed): 0.25-0.5 μg/mL for 30 min at RT
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Optimal dilution of the Nuclear marker antibody should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.2 mg/mL
-
缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
-
-
- Nuclear Marker
-
背景
- This mAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This mAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed tissue sections.
抗原
-
