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CD47 抗体

CD47 适用: 人 FACS, WB, IF, IHC, BI 宿主: 小鼠 Monoclonal B6H12-2 unconjugated
产品编号 ABIN6251897
发货至: 中国
  • 抗原 See all CD47 抗体
    CD47
    适用
    • 118
    • 45
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    宿主
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    小鼠
    克隆类型
    • 75
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    单克隆
    标记
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    This CD47 antibody is un-conjugated
    应用范围
    • 80
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    Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), BioImaging (BI)
    纯化方法
    Protein G affinity chromatography
    免疫原
    Intact CD47 purified from placenta was used as the immunogen for this antibody.
    克隆位点
    B6H12-2
    亚型
    IgG1 kappa
    Top Product
    Discover our top product CD47 Primary Antibody
  • 应用备注
    Functional Activity (Blocking): Order BSA/Azide-free format

    Flow Cytometry: 0.5-1 μg/million cells

    IF: 1-2 μg/mL

    IHC (FFPE): 0.5-1 μg/mL for 30 min at RT

    Western blot: 2-5 μg/mL

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 mg/mL
    缓冲液
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原
    CD47
    别名
    CD47 (CD47 产品)
    别名
    IAP antibody, MER6 antibody, OA3 antibody, 9130415E20Rik antibody, AA407862 antibody, AI848868 antibody, AW108519 antibody, B430305P08Rik antibody, Itgp antibody, CD47/IAP antibody, alkaline phosphatase, intestinal antibody, CD47 molecule antibody, CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antibody, Cd47 molecule antibody, ALPI antibody, CD47 antibody, Cd47 antibody
    背景
    This antibody reacts with the Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50  kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
    基因ID
    961
    UniProt
    Q08722
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