CD47 抗体
Quick Overview for CD47 抗体 (ABIN6251897)
抗原
See all CD47 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Intact CD47 purified from placenta was used as the immunogen for this antibody.
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亚型
- IgG1 kappa
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anti-CD47 (CD47) (AA 18-135) antibody
CD47 适用: 人 FACS, IHC, IF, ELISA, StM, Coat 宿主: 小鼠 Monoclonal CD47-3019 unconjugated
anti-CD47 (CD47) antibodyCD47 适用: 人, 小鼠 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal B6H12-2 unconjugated
anti-CD47 (CD47) (AA 20-140) antibodyCD47 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD47 (CD47) (AA 241-323) antibodyCD47 适用: 小鼠, 大鼠 FACS, WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD47 (CD47) (AA 18-219) antibodyCD47 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD47 (CD47) (AA 18-135) antibodyCD47 适用: 人 FACS, WB, IHC, IF, ELISA, StM, Coat 宿主: 小鼠 Monoclonal CD47-2937 unconjugated
anti-CD47 (CD47) antibody (FITC)CD47 适用: 人, Pig, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-122 FITC
anti-CD47 (CD47) antibodyCD47 适用: 人, Pig, Non-Human Primate FACS, WB, IHC (fro) 宿主: 小鼠 Monoclonal MEM-122 unconjugated
anti-CD47 (CD47) antibodyCD47 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Monoclonal 4G11 unconjugated Recombinant Antibody
anti-CD47 (CD47) (AA 44-141) antibodyCD47 适用: 人 IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Functional Activity (Blocking): Order BSA/Azide-free format
Flow Cytometry: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 min at RT
Western blot: 2-5 μg/mL
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- CD47
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别名
- CD47
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背景
- This antibody reacts with the Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
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基因ID
- 961
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UniProt
- Q08722
抗原
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