HCAM 抗体
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北京 101111
Quick Overview for HCAM 抗体 (ABIN6251853)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Stimulated human leukocytes were used as the immunogen for this CD44 / HCAM antibody.
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亚型
- IgG2a kappa
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anti-HCAM (AA 24-182) antibody
适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-HCAM antibodyCarrier-free 适用: 人, 小鼠 IHC (p) 宿主: 小鼠 Monoclonal HCAM-1097 unconjugated
anti-HCAM antibody适用: 人, 小鼠 IHC (p) 宿主: 小鼠 Monoclonal HCAM-1097 unconjugated
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应用备注
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ELISA: order BSA-free format for coating
Flow Cytometry: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCAM antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- HCAM
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背景
- This antibody recognizes a cell surface glycoprotein of 80-95 kDa, called CD44, or HCAM, on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95 kDa or hematopoietic variant (CD44-s). Higher molecular weight isoforms are described in epithelial cells (CD44-v), which are believed to function in intercellular adhesion and stromal binding. HCAM antibody immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
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基因ID
- 960
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UniProt
- P16070
抗原
-
