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S100A8/A9 Complex (Calprotectin) 抗体

This anti-S100A8/A9 Complex (Calprotectin) antibody is a 小鼠 单克隆 antibody detecting S100A8/A9 Complex (Calprotectin) in FACS, IF 和 IHC (p). Suitable for 人.
产品编号 ABIN6251842
发货至: 中国

Quick Overview for S100A8/A9 Complex (Calprotectin) 抗体 (ABIN6251842)

抗原

See all S100A8/A9 Complex (Calprotectin) (S100A8/A9) 抗体
S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This S100A8/A9 Complex (Calprotectin) antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

CAGB-426
  • 纯化方法

    PEG precipitation

    免疫原

    Recombinant human protein was used as the immunogen for the Calgranulin A + B antibody.

    亚型

    IgM kappa
  • 应用备注

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 0.5-1 μg/mL

    Immunohistochemistry (Formalin-fixed. Not suitable for frozen tissues.): 0.5-1 μg/mL for 30 min at RT (1)

    Optimal dilution of the Calgranulin A + B antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

    别名

    Calgranulin A + B

    背景

    Recognizes a 12-14  kDa doublet of Calgranulin A/B (also known as S100A8/A9 or MRP-8/14), expressed by granulocytes, monocytes and by tissue macrophages. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This mAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

    基因ID

    6280

    UniProt

    P06702

    途径

    S100 Proteins
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