Nucleoli Marker 抗体
The 小鼠 单克隆 anti-Nucleoli Marker antibody is suitable to detect Nucleoli Marker in samples from 人. It has been validated for IF, FACS, IHC 和 ICC.
产品编号 ABIN6251821
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Nucleoli Marker 抗体 (ABIN6251821)
抗原
Nucleoli Marker
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
克隆位点
NCM102-1
-
-
纯化方法
- Protein G affinity chromatography
-
免疫原
- A preparation of myeloid leukemia cell nuclei was used as the immunogen.
-
亚型
- IgG1 kappa
-
-
anti-Nucleoli Marker antibody
适用: 人 IF, FACS, ICC, IHC (p) 宿主: 小鼠 Monoclonal NM95 unconjugated
-
-
应用备注
-
Flow Cytometry: 0.5-1 μg/10^6 cells
IF: 0.5-1.0 μg/mL
Immunocytochemistry (Acetone-fixed): 0.5-1.0 μg/mL for 30 min at RT
IHC (Formalin-paraffin): 0.5-1.0 μg/mL for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Titering of the Nucleoli marker antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH 6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.2 mg/mL
-
缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
-
-
- Nucleoli Marker
-
背景
- The nucleolus is the largest structure in the nucleus of eukaryotic cells, where it primarily serves as the site of ribosome synthesis and assembly. Nucleoli also have other important functions like assembly of signal recognition particles and playing a role in the cell's response to stress. Nucleoli are made of proteins and RNA and form around specific chromosomal regions. [Wiki]
抗原
-
You are here:
