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ADIPOQ 抗体

This anti-ADIPOQ antibody is a 小鼠 单克隆 antibody detecting ADIPOQ in ELISA, IHC, FACS 和 IF. Suitable for 人.
产品编号 ABIN6251811
发货至: 中国

Quick Overview for ADIPOQ 抗体 (ABIN6251811)

抗原

See all ADIPOQ 抗体
ADIPOQ (Adiponectin (ADIPOQ))

适用

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宿主

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小鼠

克隆类型

  • 137
  • 78
单克隆

标记

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This ADIPOQ antibody is un-conjugated

应用范围

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ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

克隆位点

ADPQ-1
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    A human partial recombinant protein was used as the immunogen for this Adiponectin antibody.

    亚型

    IgG2b
  • 应用备注

    Flow Cytometry: 0.5-1 μg/10^6 cells

    IF: 1-2 μg/mL

    ELISA: order BSA free format for coating

    IHC (FFPE): 1-2 μg/mL (1)

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Adiponectin antibody to be titered up or down for optimal performance.

    1. Staining of FFPE tissue requires boiling sections in 10  mM citrate buffer,  pH 6, for 10-20 min followed by cooling at RT for 20 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    ADIPOQ (Adiponectin (ADIPOQ))

    别名

    Adiponectin

    背景

    Adiponectin (commonly called ADIPO, or by the gene name ADIPOQ) is an adipocytokine, a hormone produced in adipose tissue. It is abundantly present in plasma and has insulin like effect on glucose levels in the blood. Plasma ADIPO levels are low in insulin resistant patients who are obese, have diabetes mellitus type 2 or HIV-lipodystrophy. In women, levels tend to be higher than in men, which may be due to androgens suppressing ADIPO levels. Furthermore, adiponectin and leptin are both indicated in regulating body weight through direct action on the hypothalamus, influencing appetite. Obese people have low ADIPO levels while levels in anorexia patients are high. Adiponectin acts as ligand for various receptors, two of which have been identified, one probably involved in carbohydrate assimilation, the other in tuning the rate of metabolism.

    基因ID

    9370

    UniProt

    Q15848
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