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Alkaline Phosphatase 抗体

This anti-Alkaline Phosphatase antibody is a 小鼠 单克隆 antibody detecting Alkaline Phosphatase in IF, FACS 和 IHC (p). Suitable for 人 和 Cow.
产品编号 ABIN6251803
发货至: 中国

Quick Overview for Alkaline Phosphatase 抗体 (ABIN6251803)

抗原

See all Alkaline Phosphatase (ALP) 抗体
Alkaline Phosphatase (ALP)

适用

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人, Cow

宿主

  • 65
  • 28
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小鼠

克隆类型

  • 49
  • 48
单克隆

标记

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This Alkaline Phosphatase antibody is un-conjugated

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

ALPL-597
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    Recombinant human ALPL protein was used as the immunogen for the Alkaline Phosphatase antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 0.5-1 μg/mL

    Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT

    Prediluted format : incubate for 30 min at RT (1)

    Optimal dilution of the Alkaline Phosphatase antibody should be determined by the researcher.

    1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    Alkaline Phosphatase (ALP)

    别名

    Alkaline Phosphatase

    背景

    There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypo-phosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

    基因ID

    249

    UniProt

    P05186
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