Ku70 + Ku80 抗体
Quick Overview for Ku70 + Ku80 抗体 (ABIN6251789)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Does not react with mouse, rat, chicken
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纯化方法
- Protein G purified monoclonal antibody
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免疫原
- Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.
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亚型
- IgG1 kappa
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anti-CG18801 Gene Product From Transcript CG18801-RA (KU80) antibody (PE)
KU80 适用: 人, 灵长类 FACS, IF 宿主: 小鼠 Monoclonal KU729 PE
anti-CG18801 Gene Product From Transcript CG18801-RA (KU80) antibodyKU80 适用: 人, 灵长类 FACS, IF, ICC 宿主: 小鼠 Monoclonal KU729 unconjugated
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应用备注
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Flow cytometry: 0.5-1 μg/10e6 cells
IF: 0.5-1.0 μg/mL
Immunocytochemistry (Acetone-fixed cells): 0.25-0.5 μg/mL for 30 minutes at RT
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ku70 + Ku80 antibody to be titered up or down for optimal performance. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Ku70 + Ku80 (KU80) (CG18801 Gene Product From Transcript CG18801-RA (KU80))
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别名
- Ku70 + Ku80
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背景
- This antibody recognizes a dimer of two proteins of 70 kDa (Ku70) and ~80 kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
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基因ID
- 2547
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UniProt
- Q6FG89
抗原
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