Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
克隆位点
RB52354
抗原表位
AA 1991-2025, C-Term
纯化方法
This antibody is purified through a protein A column, followed by peptide affinity purification.
免疫原
This Mouse Med12 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1991-2025 amino acids from the C-terminal region of Mouse Med12.
MED12
适用: 小鼠, 大鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
Cy5
应用备注
WB: 1:2000. WB: 1:1000. WB: 1:2000. IHC-P: 1:25
限制
仅限研究用
状态
Liquid
缓冲液
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
有效期
6 months
抗原
MED12
(Mediator Complex Subunit 12 (MED12))
别名
Med12
背景
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity).