HSP18.5 抗体
This anti-HSP18.5 antibody is a 兔 多克隆 antibody detecting HSP18.5 in WB. Suitable for Arabidopsis thaliana.
Quick Overview for HSP18.5 抗体 (ABIN619791)
抗原
HSP18.5 (HSP18.5-C)
(18.5 kDa Class I Heat Shock Protein (HSP18.5-C))
适用
Arabidopsis thaliana
宿主
兔
克隆类型
多克隆
应用范围
Western Blotting (WB)
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交叉反应 （详细）
- Not reactive in: no confirmed exceptions from predicted reactivity are currently known
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预测反应
- dicots including: Glycine max, Medicago truncatula, Medicago sativa, Pisum sativum, Ricinus communis, Rosa chinensis, monocots including: Zea mays
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产品特性
- Expected / apparent Molecular Weight of the Antigene: 18.5 kDa
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纯化方法
- serum
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免疫原
- recombinant Arabidopsis thaliana Hsp18.5
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应用备注
- 1 : 1000 with standard ECL (WB)
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说明
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hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38°C for 3 hours at high humidity.Please, note that longer transfer time might result in losing a signal for this protein.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 200 μL of sterile water.
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储存条件
- -20 °C
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储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- HSP18.5 (HSP18.5-C) (18.5 kDa Class I Heat Shock Protein (HSP18.5-C))
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别名
- HSP18.5
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背景
- HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family. Synonymes:AtHsp18.5, 18.5 kDa heat shock protein
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分子量
- 18.5 kDa
抗原
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