ADH/ALDH 抗体
The 兔 多克隆 anti-ADH/ALDH antibody (ABIN619569) specifically detects ADH/ALDH in WB.
The antibody is reactive with Chlamydomonas reinhardtii, E. coli 和 Streptococcus pneumoniae samples.
产品编号 ABIN619569
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for ADH/ALDH 抗体 (ABIN619569)
抗原
ADH/ALDH
适用
Chlamydomonas reinhardtii, E. coli, Streptococcus pneumoniae
宿主
兔
克隆类型
多克隆
应用范围
Western Blotting (WB)
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原理
- Anti-ADH/ALDH | Alcohol/acetaldehyde dehydrogenase (bacterial/algal)
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交叉反应 （详细）
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Chlamydomonas reinhardtii, E.coli, Streptococcus pmenumoniae
Not reactive in: Lactobacillus plantarum -
预测反应
- Algae,Lactococcus lactis,Nannochloropsis gaditana,Picochlorum sp.
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纯化方法
- Serum
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免疫原
- KLH-conjugated peptide derived from available algal and bacterial ADH sequences including Chlamydomonas reinhardtii
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应用备注
- 1 : 1000 (WB)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 200 μL of sterile water
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缓冲液
- Serum
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- ADH/ALDH
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背景
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Background: Alcohol dehydrogenase (E.C.:1.1.1.1) is an important enzyme for plants and microbes. In microalgae and bacteria the conversion of Acetyl-CoA to ethanol under conditions of oxygen deprivation is catalyzed by the dual function enzyme alcohol/acetaldehyde dehydrogenase (ADH/ALDH, E.C.:1.1.1.1 /1.2.1.10). This reaction results in NAD+ recycling and allows glycolysis to proceed.
Additional information: Selected peptide is well conserved in Escherichia coli ADHE (P0A9Q7), most of the microbial dual function aldehyde/alcohol dehydrogenases (ADHE) and Iron-containing alcohol dehydrogenases are also conserved in a peptide used to elicit ADH antibody
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分子量
- 100 | 100 kDa (C.reinhardtii), 96 kDa (E.coli)
抗原
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