TUBB 抗体 (Chain beta)
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北京 101111
Quick Overview for TUBB 抗体 (Chain beta) (ABIN619536)
抗原
See all TUBB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Chain beta
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原理
- Anti-Tubulin beta chain
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交叉反应 (详细)
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Arabidopsis thaliana (including suspension cells), Chlamydomonas reinhardii, Medicago sativa
Not reactive in: Allium sativum -
预测反应
- Brassica napus,Chlamydomonas reinhardii,Glycine max,Hordeum vulgare,Micromonoas pusilla,Nicotiana attenuata,Oryza sativa,Ostreococcus lucimarinus,Picea sitcHensis,Pisum sativum,Physcomitrium patens,Populus trichocarpa,Pythium ultimum,Saccharomyces cerevisiae,Solanum tuberosum,Sorghum bicolor,Ricinus communis,Zea mays,Vigna radiata,Vitis vinifera
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纯化方法
- Immunogen affinity purified serum
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免疫原
- KLH-conjugated peptide derived from available tubulin beta chain sequences (located at a surface loop) including all Arabidopsis thaliana tubulin beta-2/beta-3 chain P29512
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应用备注
- 1 : 1000 (IF), 1 : 500 (WB)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 μL of sterile water
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- TUBB (Tubulin, beta (TUBB))
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别名
- Tubulin beta chain
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背景
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Background: Tubulin beta (TUB) together with alpha tubulin is making up microtubules.
Additional information: Please, note that tubulin beta is only detected in actively dividing meristematic cells (see immunolocalization image below). Therefore to allow detection on a western blot, analyzed material must contain enough meristematic cells with dividing activity.Signal in a western blot application in Chlamydomonas reinhardtii is obtained with a load of 100 μg/well and a dilution of 1: 500.
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分子量
- 49 | 49 kDa (Arabidopsis thaliana)
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基因ID
- 836390
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UniProt
- P29512, P24636, P29513, P29514, P29515, P29516, P29517
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途径
- Microtubule Dynamics, M Phase
抗原
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