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TUBB 抗体 (Chain beta)

The 兔 多克隆 anti-TUBB antibody (ABIN619536) specifically detects TUBB in WB 和 IF. The antibody is reactive with Alfalfa, Arabidopsis thaliana 和 Chlamydomonas reinhardtii samples.
产品编号 ABIN619536
发货至: 中国
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Quick Overview for TUBB 抗体 (Chain beta) (ABIN619536)

抗原

See all TUBB 抗体
TUBB (Tubulin, beta (TUBB))

适用

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Alfalfa, Arabidopsis thaliana, Chlamydomonas reinhardtii

宿主

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克隆类型

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多克隆

标记

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This TUBB antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF)
  • 抗原表位

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    Chain beta

    原理

    Anti-Tubulin beta chain

    交叉反应 (详细)

    Arabidopsis thaliana (including suspension cells), Chlamydomonas reinhardii, Medicago sativa
    Not reactive in: Allium sativum

    预测反应

    Brassica napus,Chlamydomonas reinhardii,Glycine max,Hordeum vulgare,Micromonoas pusilla,Nicotiana attenuata,Oryza sativa,Ostreococcus lucimarinus,Picea sitcHensis,Pisum sativum,Physcomitrium patens,Populus trichocarpa,Pythium ultimum,Saccharomyces cerevisiae,Solanum tuberosum,Sorghum bicolor,Ricinus communis,Zea mays,Vigna radiata,Vitis vinifera

    纯化方法

    Immunogen affinity purified serum

    免疫原

    KLH-conjugated peptide derived from available tubulin beta chain sequences (located at a surface loop) including all Arabidopsis thaliana tubulin beta-2/beta-3 chain P29512
  • 应用备注

    1 : 1000 (IF), 1 : 500 (WB)

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 100 μL of sterile water

    缓冲液

    Immunogen affinity purified serum in PBS pH 7.4.

    注意事项

    Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

    储存条件

    -20 °C

    储存方法

    Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • 抗原

    TUBB (Tubulin, beta (TUBB))

    别名

    Tubulin beta chain

    背景

    Background: Tubulin beta (TUB) together with alpha tubulin is making up microtubules.

    Additional information: Please, note that tubulin beta is only detected in actively dividing meristematic cells (see immunolocalization image below). Therefore to allow detection on a western blot, analyzed material must contain enough meristematic cells with dividing activity.Signal in a western blot application in Chlamydomonas reinhardtii is obtained with a load of 100 μg/well and a dilution of 1: 500.

    分子量

    49 | 49 kDa (Arabidopsis thaliana)

    基因ID

    836390

    UniProt

    P29512, P24636, P29513, P29514, P29515, P29516, P29517

    途径

    Microtubule Dynamics, M Phase
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