Biotin 抗体 (Alkaline Phosphatase (AP))
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北京 101111
Quick Overview for Biotin 抗体 (Alkaline Phosphatase (AP)) (ABIN619359)
抗原
宿主
克隆类型
标记
应用范围
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原理
- Goat anti-biotin, ALP conjugated
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纯化方法
- Immunogen affinity purified
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免疫原
- Biotin
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亚型
- IgG
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应用备注
- This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 2000 (IHC), 1 : 50 000-1 : 5 000 (WB)
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Immunogen affinity purified goat IgG.
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注意事项
- Prepare working dilution prior to use and discard after use. Do not store working dilution
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储存条件
- 4 °C,-20 °C
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储存方法
- Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
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有效期
- 48 months
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- Biotin
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物质类
- Chemical
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背景
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Background: Goat anti-biotin is a secondary antibody conjugated to ALP, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin.
Additional information: Purity of this preparation is > 95 % based on SDS-PAGE, Antibody concentration is 1,50 mg/mL (E 1 % at 280 nm = 13,0), Antibody is supplied in 30 mM triethanolamine, pH 7,2,5 mM magnesium chloride, 0,1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative of bacterial growth
抗原
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