ACTH 抗体 (N-Term) (CF®647)
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Quick Overview for ACTH 抗体 (N-Term) (CF®647) (ABIN6190912)
抗原
See all ACTH 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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原理
- Mouse Monoclonal anti-ACTH (57), CF647 Conjugate
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产品特性
- ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH), does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- N-terminal fragment of human ACTH conjugated to KLH
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亚型
- IgG1 kappa
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anti-Adrenocorticotropic hormone (ACTH) antibody
ACTH 适用: 人 ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 3A4H4 unconjugated
anti-Adrenocorticotropic hormone (ACTH) antibodyACTH 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 1A9C6 unconjugated
anti-Adrenocorticotropic hormone (ACTH) (AA 1-39) antibodyACTH 适用: 人, 大鼠 ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Adrenocorticotropic hormone (ACTH) (AA 18-39) antibodyACTH 适用: 大鼠 ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Adrenocorticotropic hormone (ACTH) antibodyACTH 适用: 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistology (formalin)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- ELISA For coating, order Ab without BSA
- Predicted to show broad species reactivity
- Optimal dilution for a specific application should be determined by user
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说明
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Normal pituitary gland or pituitary tumor
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- ACTH (Adrenocorticotropic hormone (ACTH))
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别名
- ACTH (Adrenocorticotrophic Hormone)
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物质类
- Hormone
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背景
- Adrenocorticotropin, alpha or beta or gamma Melanocyte Stimulating Hormone (MSH) or Melanotropin, beta-Endorphin, beta or gamma Lipotropin (LPH), CLIP, Met Enkephalin, POC, POMC
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分子量
- ACTH is ~5 kDa, and the POMC precursor is ~30 kDa. The molecular weight of POMC depends upon isoform variation and post-translational modifications.
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基因ID
- 5443, 1897
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UniProt
- P01189
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途径
- Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity
抗原
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