MAML2 抗体 (CF®488A)
Quick Overview for MAML2 抗体 (CF®488A) (ABIN6187050)
抗原
See all MAML2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-MAML2 (MAML2/1302), CF488A Conjugate
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产品特性
- MAML2 (mastermind-like protein 2), also known as MAM2, MAM3 or MLLMAML2, is a nuclear speckle protein that acts as a transcriptional co-activator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML2 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML2 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J?, a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML2 is essential for proper Notch binding, the C-terminal domain of MAML2 is essential for transcriptional activation. A chromosomal aberration involving the gene encoding MAML2 is implicated in mucoepidermoid carcinomas, clear cell hidradenomas and benign Warthin tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human full-length MAML2
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亚型
- IgG2a
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anti-Mastermind-Like 2 (MAML2) antibody
MAML2 适用: 人 WB, IF, IHC, FACS, ISt, StM 宿主: 小鼠 Monoclonal MAML2-1302 unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 347-506) antibodyMAML2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 796-894) antibodyMAML2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4A1 unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 780-814) antibodyMAML2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB52651 unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 750-850) antibodyMAML2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 324-373) antibodyMAML2 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 169-218) antibodyMAML2 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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MCF-7 cells. Pancreas or Placenta. Bladder or Colon Carcinoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- MAML2 (Mastermind-Like 2 (MAML2))
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别名
- MAML2
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背景
- Mam-2, MAML2, Mastermind-like protein 2
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分子量
- 125 kDa
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基因ID
- 84441
抗原
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