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Villin 1 抗体 (AA 179-311) (Biotin)

VIL1 适用: 人 WB, IHC, IF, FACS 宿主: 小鼠 Monoclonal VIL1-1314 Biotin
产品编号 ABIN6186943
发货至: 中国
  • 抗原 See all Villin 1 (VIL1) 抗体
    Villin 1 (VIL1)
    抗原表位
    • 15
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 179-311
    适用
    • 73
    • 25
    • 19
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    宿主
    • 48
    • 25
    小鼠
    克隆类型
    • 38
    • 35
    单克隆
    标记
    • 46
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Villin 1 antibody is conjugated to Biotin
    应用范围
    • 43
    • 25
    • 16
    • 16
    • 12
    • 8
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    原理
    Mouse Monoclonal anti-Villin (VIL1/1314), Biotin Conjugate
    产品特性
    This antibody recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    免疫原
    Recombinant human Villin fragment of 133 amino acid residues (aa179-311) (exact sequence is proprietary)
    克隆位点
    VIL1-1314
    亚型
    IgG1 kappa
    Top Product
    Discover our top product VIL1 Primary Antibody
  • 应用备注
    Flow cytometry 0.5-1 μg/million cells in 0.1 mL
    • Immunohistology (formalin) 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Western blotting 1-2 μg/mL
    • Immunofluorescence 1-2 μg/mL
    • Optimal dilution for a specific application should be determined by user
    说明

    A549, HepG2 and HCT116 cells. Colon or Rectum.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    100 μg/mL
    缓冲液
    PBS/0.1 % BSA/0.05 % azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原
    Villin 1 (VIL1)
    别名
    Villin (VIL1 产品)
    别名
    D2S1471 antibody, VIL antibody, Vil antibody, vil1 antibody, MGC64339 antibody, VIL1 antibody, vil1l antibody, zgc:55779 antibody, villin 1 antibody, villin 1 S homeolog antibody, VIL1 antibody, Vil1 antibody, vil1.S antibody, vil1 antibody
    背景
    VIL1, Villin-1, Villin1
    分子量
    93 kDa
    基因ID
    7429, 654595
    UniProt
    P09327
    途径
    EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
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