Villin 1 抗体 (AA 179-311) (CF®594)
Quick Overview for Villin 1 抗体 (AA 179-311) (CF®594) (ABIN6186938)
抗原
See all Villin 1 (VIL1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 179-311
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原理
- Mouse Monoclonal anti-Villin (VIL1/1314), CF594 Conjugate
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产品特性
- This antibody recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human Villin fragment of 133 amino acid residues (aa179-311) (exact sequence is proprietary)
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亚型
- IgG1 kappa
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anti-Villin 1 (VIL1) (AA 1-320) antibody
VIL1 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Villin 1 (VIL1) (N-Term) antibodyVIL1 适用: 人 WB, IF, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Villin 1 (VIL1) (AA 1-209) antibodyVIL1 适用: 人 IHC, WB, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 3E5G11 unconjugated
anti-Villin 1 (VIL1) (AA 1-209) antibodyVIL1 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 5E3B2 unconjugated
anti-Villin 1 (VIL1) (AA 180-207), (N-Term) antibodyVIL1 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB17824 unconjugated
anti-Villin 1 (VIL1) (AA 1-421) antibodyVIL1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Villin 1 (VIL1) (AA 179-311) antibodyVIL1 适用: 人 IHC, WB, StM 宿主: 小鼠 Monoclonal VIL1-1314 unconjugated
anti-Villin 1 (VIL1) (AA 1-421) antibodyVIL1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2F10 unconjugated
anti-Villin 1 (VIL1) (AA 179-311) antibodyVIL1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rVIL1-1325 unconjugated Recombinant Antibody
anti-Villin 1 (VIL1) (AA 600-700) antibodyVIL1 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-459R unconjugated Recombinant Antibody
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应用备注
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Flow cytometry 0.5-1 μg/million cells in 0.1 mL
- Immunohistology (formalin) 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Western blotting 1-2 μg/mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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A549, HepG2 and HCT116 cells. Colon or Rectum.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- Villin 1 (VIL1)
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别名
- Villin
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背景
- VIL1, Villin-1, Villin1
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分子量
- 93 kDa
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基因ID
- 7429, 654595
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UniProt
- P09327
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途径
- EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
抗原
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