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Recombinant CD235a/GYPA 抗体 (Biotin)

This Biotin-conjugated 兔 单克隆 anti-CD235a/GYPA antibody (Clone GYPA-1725R) specifically detects CD235a/GYPA in FACS, IHC 和 IF. The antibody is reactive with 人 samples.
产品编号 ABIN6184777
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Recombinant CD235a/GYPA 抗体 (Biotin) (ABIN6184777)

抗原

See all CD235a/GYPA (GYPA) 抗体
CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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单克隆

标记

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This CD235a/GYPA antibody is conjugated to Biotin

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

克隆位点

GYPA-1725R
  • 原理

    Recombinant Rabbit Monoclonal anti-Glycophorin-A / CD235a (GYPA/1725R), Biotin Conjugate

    产品特性

    This antibody recognizes a sialoglycoprotein of 39 kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant human full-length Glycophorin A protein

    亚型

    IgG kappa
  • 应用备注

    Immunohistology (formalin) 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user

    说明

    Erythrocytes in any human tissues

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原

    CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

    别名

    Glycophorin-A / CD235a

    背景

    Blood group--MN locus, GPA, GPErik, GpMiIII, GPSAT, GYPA, MN sialoglycoprotein, MNS, PAS2, Sialoglycoprotein alpha

    分子量

    39 kDa

    基因ID

    2993, 2994

    UniProt

    P02724

    途径

    Maintenance of Protein Location
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