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GFAP 抗体 (CF®594)

The CF®594-conjugated 小鼠 单克隆 anti-GFAP antibody (Clone GA-5) (ABIN6180668) specifically detects GFAP in WB, IF, FACS 和 IHC (f). The antibody is reactive with 人, 大鼠, 小鼠, Cow, Pig, 小鸡 和 兔 samples.
产品编号 ABIN6180668
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for GFAP 抗体 (CF®594) (ABIN6180668)

抗原

See all GFAP 抗体
GFAP (Glial Fibrillary Acidic Protein (GFAP))

适用

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人, 大鼠, 小鼠, Cow, Pig, 小鸡, 兔

宿主

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小鼠

克隆类型

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单克隆

标记

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This GFAP antibody is conjugated to CF®594

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

GA-5
  • 原理

    Mouse Monoclonal anti-GFAP (GA-5), CF594 Conjugate

    产品特性

    This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    GFAP isolated from pig spinal cord

    亚型

    IgG1
  • 应用备注

    Immunohistology formalin-fixed 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    Brain or Astrocytoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    GFAP (Glial Fibrillary Acidic Protein (GFAP))

    别名

    GFAP

    背景

    Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

    分子量

    ~50 kDa

    基因ID

    2670, 514227

    UniProt

    P14136
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