SOX2 抗体 (AA 176-305) (CF®594)
Quick Overview for SOX2 抗体 (AA 176-305) (CF®594) (ABIN6180440)
抗原
See all SOX2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 176-305
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原理
- Mouse Monoclonal anti-SOX2 (SOX2/1791), CF594 Conjugate
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产品特性
- SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. At present, 30 Sox genes have been identified. SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human SOX2 protein fragment (aa176-305) (exact sequence is proprietary)
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亚型
- IgG2b kappa
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anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody
KO Validated SOX2 适用: 人 WB, IF, FACS, ICC, IHC (p), IP, IHC (fro), CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) (AA 227-317) antibodySOX2 适用: 人, 小鼠, 大鼠, 山羊 WB, ELISA, FACS, ICC, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) (AA 1-140) antibodySOX2 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) (AA 1-317) antibodySOX2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) (AA 176-305) antibodySOX2 适用: 人, 小鼠 WB, ELISA, IHC, Coat, StM 宿主: 小鼠 Monoclonal SOX2-1791 unconjugated
anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) (N-Term) antibodySOX2 适用: 人, 小鼠, 大鼠, 山羊, Cow, Pig, 绵羊, 斑马鱼, 马, 兔, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibodySOX2 适用: 人 ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 10F10 unconjugated
anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) (AA 89-119), (N-Term) antibodySOX2 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB14026 unconjugated
anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) (Middle Region) antibodySOX2 适用: 人, 小鼠, 大鼠, 山羊, Cow, 绵羊, 斑马鱼, 马, 兔, 犬, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) (AA 89-119) antibodySOX2 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistology (formalin) 0.5-1 μg/mL
- ELISA 2-4 μg/mL for coating (order Ab without BSA)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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MCF-7 cells or Cervical carcinoma
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))
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别名
- SOX2
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背景
- ANOP3, Delta EF2a, MCOPS3 (Microphthalmia Syndromic type 3), SOX-2, SRY (sex determining region Y) box 2, SRY related HMG box 2, Transcription factor SOX-2, ysb
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分子量
- 34 kDa
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基因ID
- 6657, 518438
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UniProt
- P48431
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途径
- Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis
抗原
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