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Serotonin Receptor 5A 抗体 (AA 17-34)

This anti-Serotonin Receptor 5A antibody is a 兔 多克隆 antibody detecting Serotonin Receptor 5A in WB, IF, IHC, ICC 和 IEM. Suitable for 大鼠 和 Clams (Mytilus). This Primary Antibody has been cited in 2+ publications.
产品编号 ABIN617921
发货至: 中国

Quick Overview for Serotonin Receptor 5A 抗体 (AA 17-34) (ABIN617921)

抗原

See all Serotonin Receptor 5A (HTR5A) 抗体
Serotonin Receptor 5A (HTR5A)

适用

  • 33
  • 8
  • 6
  • 5
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
大鼠, Clams (Mytilus)

宿主

  • 35
  • 2

克隆类型

  • 35
  • 2
多克隆

标记

  • 30
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Serotonin Receptor 5A antibody is un-conjugated

应用范围

  • 20
  • 19
  • 9
  • 9
  • 6
  • 3
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM)
  • 抗原表位

    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 17-34

    交叉反应

    小鼠, 大鼠

    产品特性

    The 5-HT 5A Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 17-34 of the rat 5-HT5A receptor coupled to carrier protein with glutaraldehyde. The 5-HT5A Receptor was quality control tested using standard immunohisto-chemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100 - 1/300 in PBS/0.3 % Triton X-100 - Cy3 and 1/200 - 1/400 in PBS/0.3 % Triton X-100 - Bn/Av-HRP. Intensification methods such as nickel will approximately double the dilution factor as recommended. The antibody was characterized by immunoblotting and immunohistochemistry. Immunoblots of rat brain extracts revealed the presence of two bands at molecular weights of 41 and 47 kD. The lower weight band agrees with the calculated molecular weight based on amino acid sequence. The higher weight may represent glycosylated receptor protein. Immunohistochemical staining of rat brain correlates well with Northern blot analysis and in situ hybridization studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT5A receptor (17-34). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat and mouse 5-HT5A receptor.

    纯化方法

    Affinity Purified

    免疫原

    Rat 5-HT5A receptor (17-34)

    亚型

    IgG
  • 限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Contains ≤ 0.09 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C/-20 °C

    储存方法

    After reconstitution, use immediately or refrigerate at 2 - 8 °C up to 2 days. For long-term storage aliquot antibody and freeze at -15 °C or lower. Avoid repeated freeze/thaw cycles.
  • Kreiling, Stephens, Reinisch: "A mixture of environmental contaminants increases cAMP-dependent protein kinase in Spisula embryos." in: Environmental toxicology and pharmacology, Vol. 19, Issue 1, pp. 9-18, (2011) (PubMed).

    Doly, Fischer, Brisorgueil, Vergé, Conrath: "5-HT5A receptor localization in the rat spinal cord suggests a role in nociception and control of pelvic floor musculature." in: The Journal of comparative neurology, Vol. 476, Issue 4, pp. 316-29, (2004) (PubMed).

  • 抗原

    Serotonin Receptor 5A (HTR5A)

    背景

    Other Names:
    5-hydroxytryptamine receptor 5A, 5-HT5A, REC17,5ht5a,MR22,5-hydroxytryptamine (serotonin) receptor 5A, G protein-coupled

    基因ID

    25689

    途径

    JAK/STAT Signaling
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