Serotonin Receptor 5A 抗体 (AA 17-34)
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北京 101111
Quick Overview for Serotonin Receptor 5A 抗体 (AA 17-34) (ABIN617921)
抗原
See all Serotonin Receptor 5A (HTR5A) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 17-34
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交叉反应
- 小鼠, 大鼠
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产品特性
- The 5-HT 5A Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 17-34 of the rat 5-HT5A receptor coupled to carrier protein with glutaraldehyde. The 5-HT5A Receptor was quality control tested using standard immunohisto-chemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100 - 1/300 in PBS/0.3 % Triton X-100 - Cy3 and 1/200 - 1/400 in PBS/0.3 % Triton X-100 - Bn/Av-HRP. Intensification methods such as nickel will approximately double the dilution factor as recommended. The antibody was characterized by immunoblotting and immunohistochemistry. Immunoblots of rat brain extracts revealed the presence of two bands at molecular weights of 41 and 47 kD. The lower weight band agrees with the calculated molecular weight based on amino acid sequence. The higher weight may represent glycosylated receptor protein. Immunohistochemical staining of rat brain correlates well with Northern blot analysis and in situ hybridization studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT5A receptor (17-34). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat and mouse 5-HT5A receptor.
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纯化方法
- Affinity Purified
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免疫原
- Rat 5-HT5A receptor (17-34)
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亚型
- IgG
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Contains ≤ 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- After reconstitution, use immediately or refrigerate at 2 - 8 °C up to 2 days. For long-term storage aliquot antibody and freeze at -15 °C or lower. Avoid repeated freeze/thaw cycles.
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: "A mixture of environmental contaminants increases cAMP-dependent protein kinase in Spisula embryos." in: Environmental toxicology and pharmacology, Vol. 19, Issue 1, pp. 9-18, (2011) (PubMed).
: "5-HT5A receptor localization in the rat spinal cord suggests a role in nociception and control of pelvic floor musculature." in: The Journal of comparative neurology, Vol. 476, Issue 4, pp. 316-29, (2004) (PubMed).
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: "A mixture of environmental contaminants increases cAMP-dependent protein kinase in Spisula embryos." in: Environmental toxicology and pharmacology, Vol. 19, Issue 1, pp. 9-18, (2011) (PubMed).
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- Serotonin Receptor 5A (HTR5A)
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背景
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Other Names:
5-hydroxytryptamine receptor 5A, 5-HT5A, REC17,5ht5a,MR22,5-hydroxytryptamine (serotonin) receptor 5A, G protein-coupled -
基因ID
- 25689
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途径
- JAK/STAT Signaling
抗原
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