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CNN1 抗体 (Biotin)

This anti-CNN1 antibody is a 小鼠 单克隆 antibody detecting CNN1 in FACS, IF 和 IHC (f). Suitable for 人 和 大鼠.
产品编号 ABIN6178241
发货至: 中国

Quick Overview for CNN1 抗体 (Biotin) (ABIN6178241)

抗原

See all CNN1 抗体
CNN1 (Calponin 1 (CNN1))

适用

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人, 大鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This CNN1 antibody is conjugated to Biotin

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

CALP
  • 原理

    Mouse Monoclonal anti-Calponin-1 (CALP), Biotin Conjugate

    产品特性

    Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Crude human uterus extract

    亚型

    IgG1 kappa
  • 应用备注

    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    Myoepithelial cells in breast ducts

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原

    CNN1 (Calponin 1 (CNN1))

    别名

    Calponin-1

    背景

    Calponin 1 basic smooth muscle, Calponin H1 smooth muscle, Calponin-1, CNN1, Cnn1, Sm Calp, SMCC

    分子量

    34 kDa

    基因ID

    1264, 465929

    UniProt

    P51911
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