CD45RA 抗体 (CF®555)
Quick Overview for CD45RA 抗体 (CF®555) (ABIN6177158)
抗原
See all CD45RA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-CD45RA (158-4D3), CF555 Conjugate
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产品特性
- This antibody recognizes a protein of 205 kDa-220 kDa, identified as CD45RA [Workshop V, Code CD45.38]. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50 % of peripheral CD4 T-cells, 50 % of peripheral CD8 T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4 or CD8 T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO ve) from B cell lymphomas (CD45RA ve). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Stimulated human leukocytes
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亚型
- IgG2a kappa
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anti-CD45RA (CD45RA) antibody
CD45RA 适用: 人 FACS, IHC (p), WB, IP, MS 宿主: 小鼠 Monoclonal MEM-56 unconjugated
anti-CD45RA (CD45RA) antibody (PE)CD45RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-56 PE
anti-CD45RA (CD45RA) antibodyCD45RA 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-472M unconjugated
anti-CD45RA (CD45RA) antibody (FITC)CD45RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-56 FITC
anti-CD45RA (CD45RA) antibody (APC)CD45RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-56 APC
anti-CD45RA (CD45RA) antibodyGMP Grade CD45RA 适用: 人 FACS 宿主: 小鼠 Monoclonal OTH-74D4 unconjugated
anti-CD45RA (CD45RA) antibody (FITC)CD45RA 适用: 人 FACS, IHC, IHC (p), IP, IHC (fro) 宿主: 小鼠 Monoclonal FITC
anti-CD45RA (CD45RA) antibodyCD45RA 适用: 人 FACS, IHC 宿主: 小鼠 Monoclonal B-C15 unconjugated
anti-CD45RA (CD45RA) antibody (Biotin)CD45RA 适用: 人 FACS, IHC (p), WB, IP 宿主: 小鼠 Monoclonal MEM-56 Biotin
anti-CD45RA (CD45RA) antibody (PerCP)CD45RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-56 PerCP
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应用备注
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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Daudi, Raji, IM9 and human lymphocytes. Human lymph nodes and tonsils.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- CD45RA
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别名
- CD45RA
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背景
- B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
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分子量
- 205-220 kDa
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基因ID
- 5788, 654514
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UniProt
- P08575
抗原
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