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Cytokeratin 19 抗体 (CF®647)

This anti-Cytokeratin 19 antibody is a 小鼠 单克隆 antibody detecting Cytokeratin 19 in IF, FACS 和 IHC (f). Suitable for 人 和 小鼠.
产品编号 ABIN6172102
发货至: 中国

Quick Overview for Cytokeratin 19 抗体 (CF®647) (ABIN6172102)

抗原

See all Cytokeratin 19 (KRT19) 抗体
Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

适用

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小鼠

克隆类型

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单克隆

标记

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This Cytokeratin 19 antibody is conjugated to CF®647

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

KRT19-799 KRT19-800
  • 原理

    Mouse Monoclonal anti-Cytokeratin 19 (KRT19/799 + KRT19/800), CF647 Conjugate

    产品特性

    This antibody recognizes a protein of 40 kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50 % -60 % of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant full-length human KRT19 protein

    亚型

    IgG1, IgG2a kappa
  • 应用备注

    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    MCF-7, HeLa cells, Breast Cancer

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

    别名

    Cytokeratin 19

    背景

    K19, k1cs, Keratin 19 Keratin Type i 40kD, krt19

    分子量

    40 kDa

    基因ID

    3880, 654568

    UniProt

    P08727
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