ICAM-3/CD50 抗体 (CF®647)
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- 抗原 See all ICAM-3/CD50 (ICAM3) 抗体
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ICAM-3/CD50 antibody is conjugated to CF®647
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 原理
- Mouse Monoclonal anti-CD50 (ICAM-3) (186-2G9), CF647 Conjugate
- 产品特性
- This antibody recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Stimulated human leukocytes
- 克隆位点
- 186-2G9
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product ICAM3 Primary Antibody
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- 应用备注
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Blocks binding of CD11a LFA-1 to CD50 ICAM-3
- Optimal dilution for a specific application should be determined by user
- 说明
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HL-60 or THP-1 cells. Lymph node and tonsil
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light
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- 抗原
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
- 别名
- CD50 (ICAM-3) (ICAM3 产品)
- 别名
- CD50 antibody, CDW50 antibody, ICAM-R antibody, ICAM-3 antibody, ICAM3 antibody, intercellular adhesion molecule 3 antibody, ICAM3 antibody, Icam3 antibody
- 背景
- ICAMR, Intercellular adhesion molecule 3 (ICAM3)
- 分子量
- 110-160 kDa
- 基因ID
- 3385, 354563
- UniProt
- P32942
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