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ICAM-3/CD50 抗体 (CF®647)

ICAM3 适用: 人 FACS, IF, IHC (f) 宿主: 小鼠 Monoclonal 186-2G9 CF®647
产品编号 ABIN6169442
发货至: 中国
  • 抗原 See all ICAM-3/CD50 (ICAM3) 抗体
    ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
    适用
    • 110
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    宿主
    • 66
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    小鼠
    克隆类型
    • 70
    • 40
    单克隆
    标记
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    This ICAM-3/CD50 antibody is conjugated to CF®647
    应用范围
    • 59
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    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    原理
    Mouse Monoclonal anti-CD50 (ICAM-3) (186-2G9), CF647 Conjugate
    产品特性
    This antibody recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    免疫原
    Stimulated human leukocytes
    克隆位点
    186-2G9
    亚型
    IgG2b kappa
    Top Product
    Discover our top product ICAM3 Primary Antibody
  • 应用备注
    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Blocks binding of CD11a LFA-1 to CD50 ICAM-3
    • Optimal dilution for a specific application should be determined by user
    说明

    HL-60 or THP-1 cells. Lymph node and tonsil

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    100 μg/mL
    缓冲液
    PBS/0.1 % BSA/0.05 % azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Protect from light
  • 抗原
    ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
    别名
    CD50 (ICAM-3) (ICAM3 产品)
    别名
    CD50 antibody, CDW50 antibody, ICAM-R antibody, ICAM-3 antibody, ICAM3 antibody, intercellular adhesion molecule 3 antibody, ICAM3 antibody, Icam3 antibody
    背景
    ICAMR, Intercellular adhesion molecule 3 (ICAM3)
    分子量
    110-160 kDa
    基因ID
    3385, 354563
    UniProt
    P32942
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