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alpha Fetoprotein 抗体 (Biotin)

This anti-alpha Fetoprotein antibody is a 小鼠 单克隆 antibody detecting alpha Fetoprotein in IF, FACS 和 IHC (f). Suitable for 人, Pig, 犬 和 猴.
产品编号 ABIN6168513
发货至: 中国

Quick Overview for alpha Fetoprotein 抗体 (Biotin) (ABIN6168513)

抗原

See all alpha Fetoprotein (AFP) 抗体
alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

适用

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人, Pig, 犬, 猴

宿主

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小鼠

克隆类型

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单克隆

标记

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This alpha Fetoprotein antibody is conjugated to Biotin

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

C3
  • 原理

    Mouse Monoclonal anti-AFP (Alpha-Fetoprotein) (C3), Biotin Conjugate

    产品特性

    This antibody recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Alpha fetoprotein (AFP) purified from serum of a hepatoma patient

    亚型

    IgG2a kappa
  • 应用备注

    Immunohistology formalin-fixed 1-2 μg/mL
    • Does not react with cow, dog, mouse, or rat
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user

    说明

    Hep-G2 cells. Fetal liver or hepatocellular carcinoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原

    alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

    别名

    AFP (Alpha-Fetoprotein)

    背景

    Alpha fetoglobulin, FETA, HPAFP

    分子量

    70 kDa

    基因ID

    174, 518808

    UniProt

    P02771

    途径

    C21-Steroid Hormone Metabolic Process
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