alpha Fetoprotein 抗体 (CF®405S)
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- 抗原 See all alpha Fetoprotein (AFP) 抗体
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This alpha Fetoprotein antibody is conjugated to CF®405S
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 原理
- Mouse Monoclonal anti-AFP (Alpha-Fetoprotein) (C2), CF405S Conjugate
- 产品特性
- This antibody recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Alpha fetoprotein (AFP) purified from serum of a hepatoma patient
- 克隆位点
- C2
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product AFP Primary Antibody
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- 应用备注
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Immunohistology formalin-fixed 1-2 μg/mL
- Does not react with cow, dog or rat, others not known
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- 说明
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Hep-G2 cells. Fetal liver or hepatocellular carcinoma
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light
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- 抗原
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
- 别名
- AFP (Alpha-Fetoprotein) (AFP 产品)
- 别名
- FETA antibody, HPAFP antibody, alpha fetoprotein antibody, alpha-fetoprotein antibody, AFP antibody, Afp antibody
- 背景
- Alpha fetoglobulin, FETA, HPAFP
- 分子量
- 70 kDa
- 基因ID
- 174, 518808
- UniProt
- P02771
- 途径
- C21-Steroid Hormone Metabolic Process
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