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s100a4 抗体 (CF®488A)

This CF®488A-conjugated 小鼠 单克隆 anti-s100a4 antibody (Clone S100A4-1481) specifically detects s100a4 in WB, IHC, IF 和 FACS. The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN6167383
发货至: 中国
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Quick Overview for s100a4 抗体 (CF®488A) (ABIN6167383)

抗原

See all s100a4 (S100A4) 抗体
s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

适用

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人, 小鼠

宿主

  • 115
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小鼠

克隆类型

  • 93
  • 76
单克隆

标记

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This s100a4 antibody is conjugated to CF®488A

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

克隆位点

S100A4-1481
  • 原理

    Mouse Monoclonal anti-S100A4 (S100A4/1481), CF488A Conjugate

    产品特性

    S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, ?6?4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant human S100A4 protein

    亚型

    IgG1 kappa
  • 应用备注

    Immunohistology (formalin) 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    HeLa, A549 or A375 Cells. Placenta

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

    别名

    S100A4

    分子量

    10-12 kDa

    基因ID

    6275, 654444

    UniProt

    P26447

    途径

    S100 Proteins
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