MVP 抗体 (CF®640R)
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北京 101111
Quick Overview for MVP 抗体 (CF®640R) (ABIN6165351)
抗原
See all MVP 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-Major Vault Protein (MVP) (1032), CF640R Conjugate
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产品特性
- This antibody recognizes a protein of 104 kDa-110 kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70 % of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Proteins precipitated from human breast cancer MCF-7 cells
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亚型
- IgG1 kappa
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anti-Major Vault Protein (MVP) (AA 1-893) antibody
MVP 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2H3-1A6 unconjugated
anti-Major Vault Protein (MVP) (AA 2-272) antibodyMVP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Major Vault Protein (MVP) antibodyMVP 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Major Vault Protein (MVP) antibodyKO Validated MVP 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Major Vault Protein (MVP) (AA 2-259) antibodyMVP 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, FACS, IF, IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Major Vault Protein (MVP) (AA 88-180) antibodyMVP 适用: 人, 斑马鱼 ELISA, FACS, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Major Vault Protein (MVP) antibodyMVP 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Major Vault Protein (MVP) (N-Term) antibodyMVP 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Major Vault Protein (MVP) (AA 1-894) antibodyMVP 适用: 人 WB, IHC, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2H3-1A6 unconjugated
anti-Major Vault Protein (MVP) antibodyMVP 适用: 人 FACS, IF 宿主: 小鼠 Monoclonal 1014 unconjugated
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应用备注
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
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说明
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MCF-7/HeLa, breast tumors
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- MVP (Major Vault Protein (MVP))
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背景
- Lung resistance-related protein (LRP), Major Vault Protein, MVP, VAULT1
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分子量
- 104-110 kDa
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基因ID
- 9961, 632177
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UniProt
- Q14764
抗原
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