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Glypican 3 抗体 (AA 511-580) (CF®647)

Name: Glypican 3 抗体 (AA 511-580) (CF®647)
SKU: ABIN6163721
Availability: OutOfStock

This anti-Glypican 3 antibody is a 小鼠单克隆 antibody detecting Glypican 3 in IF, FACS 和 IHC (f). Suitable for 人.

产品编号 ABIN6163721

发货至: 中国

Quick Overview for Glypican 3 抗体 (AA 511-580) (CF®647) (ABIN6163721)

抗原

See all Glypican 3 (GPC3) 抗体

Glypican 3 (GPC3)

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This Glypican 3 antibody is conjugated to CF®647

应用范围

Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

GPC3-863 1G12

Discover our top product GPC3 Primary Antibody

抗原表位
21

16

12

8

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7

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5

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
AA 511-580

原理

Mouse Monoclonal anti-Glypican-3 (GPC3/863 + 1G12), CF647 Conjugate

产品特性

Glypican-3 (GPC3) is an integral membrane protein that is mutated in the Simpson-Golabi-Behmel syndrome (SGBS). SGBS is characterized by pre- and post-natal overgrowth and is a recessive X-linked condition. GPC3 may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilm's tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression has also been found in some types of embryonal tumors, such as Wilm's tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100 % in follicular carcinoma and 70 % in papillary carcinoma. Expression of GPC3 in follicular carcinoma was significantly higher than that of follicular adenoma. In contrast, GPC 3 is not expressed in anaplastic carcinoma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

免疫原

Recombinant fragment containing amino acids 511-580 of human glypican-3 (1G12), Recombinant full-length human GPC3 protein (GPC3/863)

亚型

IgG
anti-Glypican 3 (GPC3) antibody
GPC3 适用: 人, 大鼠, 小鼠 FACS, WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 9C2 unconjugated

anti-Glypican 3 (GPC3) (AA 344-559) antibody
GPC3 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Glypican 3 (GPC3) antibody
GPC3 适用: 人, 大鼠 IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal GPC3-863 unconjugated

anti-Glypican 3 (GPC3) (AA 55-200) antibody
GPC3 适用: 人 IF, FACS, WB, IHC, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 9C2 unconjugated

anti-Glypican 3 (GPC3) (AA 359-554) antibody
GPC3 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 2C7E9 unconjugated

anti-Glypican 3 (GPC3) (AA 55-200) antibody
GPC3 适用: 人, 小鼠 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 2A2C3 unconjugated

anti-Glypican 3 (GPC3) antibody
GPC3 适用: 人, 大鼠 IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal rGPC3-863 unconjugated Recombinant Antibody

anti-Glypican 3 (GPC3) (AA 511-580) antibody
GPC3 适用: 人 IF, FACS, WB, IHC, StM 宿主: 小鼠 Monoclonal 1G12 unconjugated

anti-Glypican 3 (GPC3) (AA 359-554) antibody
GPC3 适用: 人, 大鼠 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 4D5B1 unconjugated

anti-Glypican 3 (GPC3) antibody
GPC3 适用: 人, 小鼠 IF, WB, IHC (p), EIA 宿主: 小鼠 Monoclonal 9C2 unconjugated

应用备注
Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
说明

293T cells or Hepatocellular carcinoma

限制

仅限研究用
状态

Liquid

浓度

100 μg/mL

缓冲液

PBS/0.1 % BSA/0.05 % azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Protect from light
抗原

Glypican 3 (GPC3)

别名

Glypican-3

背景

DGSX, Glypican proteoglycan 3, GPC3, GTR2-2, Heparan sulphate proteoglycan, Intestinal protein OCI-5, MXR7, OCI-5, SDYS, Secreted glypican-3, SGBS1

分子量

67 kDa

基因ID

2719, 644108

UniProt

P51654

途径

Glycosaminoglycan Metabolic Process