Clathrin, Light Chain (N-Term) 抗体 (Biotin)
适用: 人
WB, IHC, FACS, IF
宿主: 小鼠
Monoclonal
CLC-1421
Biotin
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- 抗原
- Clathrin, Light Chain
- 抗原表位
- N-Term
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- Biotin
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- 原理
- Mouse Monoclonal anti-Clathrin, Light chain (CLC/1421), Biotin Conjugate
- 产品特性
- This antibody recognizes proteins of 31-44 kDa, which are identified as Clathrin Light Chains (both A & B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60 % homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Purified recombinant N-terminal fragment of human Clathrin Light Chain
- 克隆位点
- CLC-1421
- 亚型
- IgG2b kappa
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- 应用备注
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Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- 说明
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HeLa cells. Placenta or Prostate Carcinoma.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- 抗原
- Clathrin, Light Chain
- 分子量
- 31-44 kDa
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